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sonication buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher sonication buffer
    Sonication Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sonication+in+buffer/Triton+X-100/bio_rxiv__64898__2026__04__30__721885-222-8-27
    Average 99 stars, based on 1 article reviews
    sonication buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sonication:

    Article Title: Regulation of Estrogen Sulfotransferase Expression by Confluence of MCF10A Breast Epithelial Cells: Role of the Aryl Hydrocarbon Receptor
    Article Snippet: .. Cells were pelleted and homogenized by sonication in buffer (200 μl/flask) consisting of 50 mM Tris-HCl, 25 mM sucrose, 1 mM EDTA, and 1× Halt protease inhibitor (Thermo Fisher Scientific, Waltham, MA), pH 7.4. ..

    Article Title: Expression of estrogenicity genes in a lineage cell culture model of human breast cancer progression
    Article Snippet: .. Cells were pelleted and homogenized by sonication in buffer (200 μl per flask) consisting of 50 mM Tris–HCl, 25 mM sucrose, 1 mM EDTA and 1× Halt protease inhibitor (Thermo Fisher Scientific, Rockford, IL), pH 7.4. ..

    Article Title: Case Report: Clinical application of an in vitro prenylation assay in the diagnosis of an early-onset case of mevalonate kinase deficiency harbouring a novel MVK variant
    Article Snippet: .. Briefly, cell lysates were generated from cryopreserved PBMCs by sonication in buffer (50 mM HEPES, 50 mM NaCl, 2 mM MgCl 2 , 100 μM GDP, protease inhibitor cocktail) and protein was quantified using a Pierce Rapid Gold BCA Protein Assay (Thermo Fisher Scientific). .. Unprenylated Rab proteins in cell lysates were prenylated in an in vitro reaction by incubating 10 mg protein from the lysates with 2 μM recombinant Rab geranylgeranyltransferase (GGTase II, Jena Bioscience), 2 μM Rab escort protein (Jena Bioscience), 0.5 μM biotin-tagged geranyl diphosphate (Jena Bioscience) and 2 mM DTT for 5 h at room temperature.

    Article Title: 2‐Oxoglutarate Analog‐Based Biomolecular Tools for Exploring Structure–Activity Relationships in Nonheme Iron Enzymes
    Article Snippet: .. Cells were resuspended and lysed via sonication in buffer (20 mM Tris HCl, 500 mM NaCl, and 5 mM imidazole at pH = 7.5) with Pierce Protease Inhibitor Tablets (Thermofisher Scientific). .. Cell lysate was purified using Ni-NTA affinity column (Cytiva) with running buffer (20 mM Tris HCl, 500 mM NaCl, and 5 mM imidazole at pH = 7.5) and elution buffer (20 mM Tris HCl, 100 mM NaCl, and 300 mM imidazole at pH = 7.5).

    Protease Inhibitor:

    Article Title: Regulation of Estrogen Sulfotransferase Expression by Confluence of MCF10A Breast Epithelial Cells: Role of the Aryl Hydrocarbon Receptor
    Article Snippet: .. Cells were pelleted and homogenized by sonication in buffer (200 μl/flask) consisting of 50 mM Tris-HCl, 25 mM sucrose, 1 mM EDTA, and 1× Halt protease inhibitor (Thermo Fisher Scientific, Waltham, MA), pH 7.4. ..

    Article Title: Expression of estrogenicity genes in a lineage cell culture model of human breast cancer progression
    Article Snippet: .. Cells were pelleted and homogenized by sonication in buffer (200 μl per flask) consisting of 50 mM Tris–HCl, 25 mM sucrose, 1 mM EDTA and 1× Halt protease inhibitor (Thermo Fisher Scientific, Rockford, IL), pH 7.4. ..

    Article Title: Case Report: Clinical application of an in vitro prenylation assay in the diagnosis of an early-onset case of mevalonate kinase deficiency harbouring a novel MVK variant
    Article Snippet: .. Briefly, cell lysates were generated from cryopreserved PBMCs by sonication in buffer (50 mM HEPES, 50 mM NaCl, 2 mM MgCl 2 , 100 μM GDP, protease inhibitor cocktail) and protein was quantified using a Pierce Rapid Gold BCA Protein Assay (Thermo Fisher Scientific). .. Unprenylated Rab proteins in cell lysates were prenylated in an in vitro reaction by incubating 10 mg protein from the lysates with 2 μM recombinant Rab geranylgeranyltransferase (GGTase II, Jena Bioscience), 2 μM Rab escort protein (Jena Bioscience), 0.5 μM biotin-tagged geranyl diphosphate (Jena Bioscience) and 2 mM DTT for 5 h at room temperature.

    Article Title: 2‐Oxoglutarate Analog‐Based Biomolecular Tools for Exploring Structure–Activity Relationships in Nonheme Iron Enzymes
    Article Snippet: .. Cells were resuspended and lysed via sonication in buffer (20 mM Tris HCl, 500 mM NaCl, and 5 mM imidazole at pH = 7.5) with Pierce Protease Inhibitor Tablets (Thermofisher Scientific). .. Cell lysate was purified using Ni-NTA affinity column (Cytiva) with running buffer (20 mM Tris HCl, 500 mM NaCl, and 5 mM imidazole at pH = 7.5) and elution buffer (20 mM Tris HCl, 100 mM NaCl, and 300 mM imidazole at pH = 7.5).

    Generated:

    Article Title: Case Report: Clinical application of an in vitro prenylation assay in the diagnosis of an early-onset case of mevalonate kinase deficiency harbouring a novel MVK variant
    Article Snippet: .. Briefly, cell lysates were generated from cryopreserved PBMCs by sonication in buffer (50 mM HEPES, 50 mM NaCl, 2 mM MgCl 2 , 100 μM GDP, protease inhibitor cocktail) and protein was quantified using a Pierce Rapid Gold BCA Protein Assay (Thermo Fisher Scientific). .. Unprenylated Rab proteins in cell lysates were prenylated in an in vitro reaction by incubating 10 mg protein from the lysates with 2 μM recombinant Rab geranylgeranyltransferase (GGTase II, Jena Bioscience), 2 μM Rab escort protein (Jena Bioscience), 0.5 μM biotin-tagged geranyl diphosphate (Jena Bioscience) and 2 mM DTT for 5 h at room temperature.

    Bicinchoninic Acid Protein Assay:

    Article Title: Case Report: Clinical application of an in vitro prenylation assay in the diagnosis of an early-onset case of mevalonate kinase deficiency harbouring a novel MVK variant
    Article Snippet: .. Briefly, cell lysates were generated from cryopreserved PBMCs by sonication in buffer (50 mM HEPES, 50 mM NaCl, 2 mM MgCl 2 , 100 μM GDP, protease inhibitor cocktail) and protein was quantified using a Pierce Rapid Gold BCA Protein Assay (Thermo Fisher Scientific). .. Unprenylated Rab proteins in cell lysates were prenylated in an in vitro reaction by incubating 10 mg protein from the lysates with 2 μM recombinant Rab geranylgeranyltransferase (GGTase II, Jena Bioscience), 2 μM Rab escort protein (Jena Bioscience), 0.5 μM biotin-tagged geranyl diphosphate (Jena Bioscience) and 2 mM DTT for 5 h at room temperature.



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